In the water bodies of Ukraine, 6 new species of naked amoebae were found: Saccamoeba sp., Ripella sp., Vannella lata Page, 1988, Thecamoeba sp., Acanthamoeba sp., Vahlkampfia sp. According to the current taxonomy, they belong to 3 classes, 4 orders, 5 families and 6 genera. New localities and original descriptions of the species are presented, along with brief characteristics of the corresponding genera. Thecamoeba sp. and Acanthamoeba sp. are first reported from the territory of Ukraine.