Modified Method of Metaphase Plates Obtaining for Polypoid Fish Genera Carassius and Cobitis Karyotyping (Actinopterigii, Cypriniformes)

Pukhtayevych, P. P.



Modern methods for obtaining metaphase plates from the somatic cells of fish, most effective in the study of polyploid species, were selected and tested. The technique with CoCl2 and colchicine treatment is recommended on the basis of empirical data for representatives of the genera Carassius and Cobitis. The detailed description of this modifi ed technique and characteristics of metaphase plates of Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 and triploid form of Cobitis taenia Linnaeus, 1758 received under tested method is presented in the paper.