Findings of Entomopathogenic Nematodes (Rhabditida, Steinernematidae) in Nature Reserves in Ukraine

Yakovlev, Ye. B., Kharchenko, V. A., Mrácek, Z.



Five strains of Steinernema Travassos, 1927 were isolated by live baiting method with last instar larvae of Tenebrio molitor Linnaeus, 1758 from the reserves of some central and southern oblasts of Ukraine and the Crimean AR. Entomopathogenic nematodes were recovered from 5 of 196 (2.6 %) soil samples collected in 2010. Isolated nematodes were identified using a combination of molecular (ITS1–5.8S–ITS2 rDNA gene sequencing) and morphological techniques. Four of the isolated strains were recognized as S. feltiae (Filipjev, 1934), one as S. arenarium (Artyukhovsky, 1967).